If I'm reading this right, you had one 3 shot group (out of the 3 with different charge weights) that shot well in the .243, you duplicated that load, and it didn't shoot as well the second time around?
Lots of people fall into the trap of thinking a 3 round group tells you something when in most cases it tells us very little. If a guy with a solid hunting rifle and better than average shooting abilities takes a quality box of ammo and shoots all 20 rounds at the same POA, i'd guess a lot of those groups are going to fall into 1.5-2.5 MOA range. Within that 20 shot group, there's probably a handful of combinations of different 3 shot groups that would be pretty good and a handful that would be pretty poor. Which 3 shot group you get is much more likely caused by randomness than any little powder charge or seating depth tweak.